A simple, precise and accurate method was developed for the estimation of sildenafil (SIL) and aspirin (ASP) in synthetic mixture using first order derivative spectrophotometry. Wavelengths selected for quantitation were 255.4 nm for sildenafil (zero crossing point of aspirin) and 265 nm for aspirin (zero crossing point of sildenafil). The method was validated with respect to linearity, accuracy, precision, limit of detection and limit of quantitation in accordance with the international conference on harmonization (ICH) guidelines. Linearity was observed in concentration range of 5-35 μg/ml for sildenafil and 20-80 μg/ml for aspirin. The limit of detection and limit of quantitation were found to be 0.15 μg/ml and 0.44 μg/ml for sildenafil and 1.94 μg/ml and 5.87 μg/ml for aspirin. The percentage recovery of sildenafil and aspirin was found to be 100.75% and 100.11%, respectively. The % R.S.D. values for intra-day and inter-day precision study were <2.0%, confirming that the method was precise. The method can be successfully employed for the simultaneous estimation of sildenafil and aspirin in synthetic mixture.
Loading....